Life Sciences in the 21st Century:
Agriculture Case Study: Dr. Loke Kok

 
Loke Kok
Entomology
Biological Control of Thistles
Initiation Date: Over 20 years ago
History:

A congested field taken over by Musk Thistle plants


Larvae of the leaf-eating weevil feeding within the foliage crown of the thistle.

The larvae from the weevil that attacks thistle flowers by laying eggs within the bloom, ultimately using it for food.
This project was originally funded by the livestock industry because the thistles invade pasture lands and cattle don't eat them. Thistles then begin to outcompete existing grasses and other plants necessary for grazing. The two plants which caused the concern, the Musk Thistle (Carduus thoermeri) and the Plumeless Thistle (Carduus acanthoides), are not native to the U.S. They came into the country in the mid-1800s from Europe as seeds in imported hay and grain shipments. The plants have since managed to spread to 40 of the 48 contiguous states due to a variety of reasons: the large number of seeds produced per plant, seed longevity (20 years!), exceptional competitiveness of the plant, and the lack of natural enemies.

The ineffectiveness of herbicide control methods over the past 30 years led to the belief that there has to be a better/alternative way...everything else seemed to be failing! Biological control had been untried and appeared to be ready for investigation; but it, too, would not be without its obstacles. Since biological control involves the use of another organism as a control mechanism, and since that other organism may not be native to this country, stiff federal regulations MUST be followed prior to releasing any potential biological control organism.

Two weevils (insects) were eventually identified in Europe that naturally attack these thistles in their native European fields. One weevil lays eggs in the flower buds and the other in the mid-rib of leaves; this second weevil later feeds in the crown tissues of rosettes. The result in both cases is a much weakened plant with few to no flowers and seeds, and a much decreased competitive ability to survive in the surrounding ecosystem. Another bonus is that the weevils do not attack the same part of the plant, and they attack different stages of the thistle life-cycle. Once the first weevil has attacked the plant in the rosette stage, the second weevil attacks the surviving plants in the flowering stage. Thus the two weevils complement one another....Of course some thistles remain undiscovered or else the species would die out, but the weevils keep them in check. Both weevils were eventually released into Virginia's landscape following extensive testing. Dr. Kok had to make certain that no other wild plants would be suitable hosts and he also had to determine if the weevils would survive our climate. The initial natural dispersal of the weevils was slow because there were so many thistles,....why should the weevils stray very far from the release site if there were lots of food! In fact, the VT work showed that it took almost 6 years for a weevil population to show up in Dublin after being released in Pulaski...and these two towns are right next to each other. By the mid-1980s, thistle populations within tests sites were reduced by 90% following introduction of weevils 5-10 years earlier.

Contemporary Impact of the Work: Livestock
Cattle farms now have the biological means to reduce thistle problems in grazing land much more effectively than chemical treatments.
Regional/National
Virginia Tech is now supplying weevils to requesting sites.
Commercial
Private companies are now producing the weevils for large scale requests, opening up entrepreneurial opportunities. Keep in mind that there is a limit to selective commercial exploitation of the weevils since living organisms can't be patented.

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